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Detailed description

Food Testing Project GB 5009.248-2016 National Food Safety Standard - Determination of Lutein in Foo

Detection standard

  • Detection specification


This standard specifies the liquid chromatography method for the determination of lutein in food This standard is applicable to the liquid chromatography determination of lutein in infant formula milk powder, dairy products, frozen beverages, rice flour products, baked goods, jam, jelly, and beverages


Foods with high fat content (fat content not less than 3% on a dry basis) are saponified with potassium hydroxide solution at room temperature to free lutein. Then, they are extracted with ether n-hexane cyclohexane (40+40+20, volume ratio), separated by liquid chromatography, detected by UV detector or diode array detector, and quantified by external standard method Other foods directly extract lutein from the sample with ether n-hexane cyclohexane (40+40+20, volume ratio). The extraction solution is purified by a neutral alumina solid-phase extraction column, separated by liquid phase chromatography, detected by UV detector or diode array detector, and quantified by external standard method

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