Cosmetic testing

Place 25-30mg in a 25ml colorimetric tube, add 1ml of 15% nitric acid, heat in a boiling water bath for 2 minutes, add 10ml of water, cool, and add 2 drops of 0.2% xylenol orange indicator. If bismuth compounds are present, the solution will appear reddish purple (2) EDTA-2Na titration method: Bismuth reacts with thiourea to form a yellow complex, which is unstable. When EDTA-2Na is used for titration, chromium in the complex reacts with EDTA-2Na to form a colorless complex, and the disappearance of yellow color is the reaction endpoint III. Determination of Mercury in Cosmetics&Nbsp According to the hygiene standards for cosmetics, mercury and mercury compounds cannot be used as raw materials for cosmetics. There are many methods for detecting mercury, including the reduction oxidation cold atomic absorption method (GB7917.1-87), which was established in 1987, as well as the copper iodide method and the high-temperature pyrolysis gold amalgam enrichment cold atomic absorption method, which can be quickly qualitative&Nbsp

(1) Qualitative method of cuprous iodide

1.  Application scope: This method is suitable for rapid qualitative analysis of mercury in cosmetics. The rapid method (5.1) is applicable to the detection of inorganic mercury salts in cosmetics without coal tar pigments. If the color of the test sample is difficult to identify due to the pigment content, the extraction method in 5.2 can be used&Nbsp 2 Principle: Inorganic mercury salts in cosmetics form a red mercury containing iodine complex Cu 2 HgI 4 with cuprous iodide&Nbsp (2) Catalysis Enrichment Cold Atomic Absorption Method&Nbsp

1.  Application scope: This method is applicable to the determination of mercury in cosmetics

2.  Principle: The sample is subjected to high-speed dispersion treatment under the action of surfactants, allowing oil, cream, paste, etc. to be fully mixed with water and dispersed in the prepared sample solution. The mercury in the sample solution is reduced to mercury atoms by stannous chloride in an alkaline medium and in the presence of Cu2+. Along with the interfering substances, it is carried into a high-temperature palladium catalytic tube by carrier gas. After digestion and removal of all interfering substances, the mercury is captured and absorbed by a gold film mercury enrichment tube. After release, it is measured using a mercury meter

4. Animal experiments (according to the "Hygienic Standards for Cosmetics")

Acute oral toxicity test

Acute Percutaneous Toxicity Test Acute and multiple skin irritation/corrosion tests Acute eye irritation/corrosiveness test Skin allergy/allergy test Skin phototoxicity test Subchronic percutaneous toxicity test

5. Animal replacement test (according to OECD, EU, and inspection and quarantine industry standards)

3T3 neutral red absorption phototoxicity test

Acute toxicity keratinocyte test

Acute toxicity 3T3 cell test

Eye irritation/corrosive chicken embryo allantoic membrane test

Stem cell test for embryonic and developmental toxicity

6 Adaptability and efficacy items

Human skin patch test (deodorization/sun protection/freckle removal)  Determination of sun protection factor (SPF) of sunscreen cosmetics (human trial) Determination of Sun Protection Index (SPA) of Sun Protection Cosmetics (Human Trial) Waterproof performance test of sunscreen cosmetics (human test) Efficacy evaluation of moisturizing cosmetics

Efficacy evaluation of anti oil cosmetics 

Evaluation of the efficacy of anti wrinkle cosmetics  Efficacy Evaluation of Whitening Cosmetics

Efficacy evaluation of anti hair loss and hair growth products

7. Content detection of additives such as sunscreen and preservatives

Generally, cosmetics contain preservatives that can be used to inhibit bacterial growth and reproduction, prevent spoilage, but excessive addition can also cause irritation to the human body and even cause contact dermatitis.

. Therefore, the hygiene standards for cosmetics in China limit the types and amounts of preservatives and sunscreen used in cosmetics

Detection of harmful substances such as formaldehyde, hydroquinone, phenol, etc.

The harmful substances in cosmetics mainly come from the chemical raw materials of cosmetics, as well as the prohibited and restricted chemicals added Adding sex hormones to cosmetics can promote hair growth, eliminate wrinkles, increase skin elasticity, and prevent skin aging. However, long-term use of cosmetics containing hormones can easily lead to metabolic disorders and the occurrence of cancer in the human body

As a solvent, methanol is added to perfume and hair spray products, which has obvious anesthetic effect on the central nervous system and can cause brain edema. It has a special selective effect on the optic nerve and retina, causing optic nerve atrophy and leading to binocular blindness Hydroquinone has a whitening effect on the skin and is widely used in whitening cosmetics. Due to its high toxicity and irritation, it can cause adverse reactions such as itching, allergies, redness, burns, etc. If used on a large scale for a long time, it will also have toxic effects on human organs and systems, and cause exogenous white spot disease, brown spot disease, leukemia, tumors, and cardiovascular diseases Formaldehyde has anti-corrosion, sterilization and disinfection effects, and is widely used in cosmetics. Direct contact with formaldehyde on the skin can cause allergic dermatitis, pigmentation, and necrosis. Inhaling high concentrations of formaldehyde can trigger bronchial asthma

The above article is a partial list. For more testing needs and details, please consult the online consultant of the free consulting agency: 15201733840 (phone and WeChat), and conduct testing on the top 100 inspection websites. Issuing authoritative testing reports has legal effect