Sinomenine detectionHow to apply for a report? What items need to be tested? We will conduct testing and evaluation in strict accordance with the standards. We can also provide personalized testing plans and reports according to your needs.

The detection methods of sinomenine mainly include the following:

High-performance liquid chromatography (HPLC)
Instrument: Usually a high-performance liquid chromatograph equipped with a UV detector is used, such as the high-performance liquid chromatograph of Walers Company in the United States or the Hitachi 638-50 high-performance liquid chromatograph.
Chromatographic conditions: There are various choices of chromatographic columns, such as u-BondapakCia, KromasiL-C18, etc. The mobile phase is generally acetonitrile-water-ethylenediamine or methanol-ethylenediamine system, and the detection wavelength is mostly 260nm or 262nm.
Determination steps:
Prepare reference solution and test solution. The reference solution is usually prepared by accurately weighing the sinomenine reference, dissolving it with methanol, and diluting it to a certain concentration. The test solution is obtained by taking the medicinal material powder, ultrasonically extracting it with 70% ethanol, fixing the volume, and filtering it.
The reference solution and the test solution are injected into the liquid chromatograph respectively for determination.
The content of sinomenine is calculated based on the peak area or peak height.
Capillary electrophoresis (CE)
Instrument: Use an isotachophore or capillary electrophoresis instrument, such as LKB2127 Tachophor isotachophore.
Electrophoresis conditions: The electrolyte system includes a leading electrolyte and a trailing electrolyte. The operating parameters such as the inner diameter of the capillary, the mobilization current, and the detection wavelength need to be adjusted according to the instrument and the sample.
Determination steps:
Prepare the reference solution and the test solution in a similar manner to HPLC.
Inject the solution into the capillary electrophoresis instrument for electrophoretic separation.
Calculate the content of sinomenine based on the electrophoresis spectrum and peak area.
Ultraviolet spectrophotometer (UV)
Instrument: Use an ultraviolet spectrophotometer, such as a Hitachi 200-10 ultraviolet spectrophotometer.
Determination conditions: Determine the absorbance at a specific wavelength (such as 265nm).
Determination steps:
Prepare an ethanol solution of sinomenine.
Determine the absorbance of the solution on a UV spectrophotometer.
The content of sinomenine is calculated based on the relationship between absorbance and concentration.
Other methods
In addition to the above methods, single-scan oscillographic polarography, thin-layer scanning, chemiluminescence, polarimetry, and other methods can be used to detect sinomenine. These methods have their own characteristics, and the appropriate method can be selected for determination according to actual needs and experimental conditions.
In general, there are many methods for detecting sinomenine, and the choice of which method depends on the experimental conditions, sample properties, and detection requirements. In practical applications, the method should be selected and optimized according to the specific situation to ensure the accuracy and reliability of the test results.

Test report function

1、Project bidding: issue authoritative third-party CMA/CNAS qualification report；

2、E-commerce platform entry: quality inspection reports recognized by major e-commerce platforms；

3、Used as sales report: issuing a test report with legal effect to make consumers feel more at ease；

4、Papers and scientific research: providing professional and personalized testing needs；

5、Judicial services: providing scientific, fair and accurate testing data；

6、Industrial problem diagnosis: verifying the troubleshooting and correction of problems in industrial production；