Antibacterial gel testingHow to apply for a report? What items need to be tested? We will conduct testing and evaluation in strict accordance with the standards. We can also provide personalized testing plans and reports according to your needs.

The detection methods of antibacterial gel mainly include the following:

1. OD value counting method
Steps:
Incubate a bacterial suspension with a certain bacterial liquid concentration (about 10^6 CFU/ml) with the antibacterial gel for a period of time (the time can be adjusted according to the experimental design).
After the incubation, dilute the bacterial suspension to an appropriate ratio for later counting.
The OD value counting method is used to calculate the antibacterial rate, and the OD600 absorbance value of the bacterial suspension after the antibacterial gel is directly measured.
Result judgment: The lower the OD value, the stronger the antibacterial effect.
2. Spreading plate method
Steps:
Incubate a bacterial suspension with a certain bacterial liquid concentration with the antibacterial gel.
After the incubation, dilute the bacterial suspension and inoculate it on the surface of the solid nutrient culture medium.
After culturing at 37°C for a period of time (such as 12~24 hours), take out the culture dish for photographing and counting.
Calculation of antibacterial rate:
The plate counting method can be used to calculate the antibacterial rate by taking bright field photos and using software (such as ImageJ).
The live/dead/staining method can also be used. The live and dead bacteria double staining kit is used to dye the live and dead bacteria green and red respectively, and then the live and dead bacteria are observed under a microscope to obtain a more intuitive data chart.
III. Antibacterial ring method
Steps:
Put a cylindrical hydrogel sample of a certain volume or a paper sheet soaked in an antibacterial gel solution on a solid nutrient medium evenly coated with a bacterial suspension.
After culturing at 37°C for a period of time, measure the size of the antibacterial ring.
Result judgment: The antibacterial property of the hydrogel is determined by the diameter of the antibacterial ring. The larger the diameter, the better the antibacterial property, and vice versa.
IV. Scanning electron microscopy (SEM)
Steps:
The bacteria after the hydrogel is acted on are fixed with a 2.5% glutaraldehyde solution at 4°C for a period of time (such as 40 minutes).
After gradient dehydration using ethanol aqueous solutions with concentrations of 20% to , the sample is dried.
Perform SEM observation to observe the morphological changes of bacteria.
Result determination: It can be seen intuitively from the SEM image that the bacterial cell membrane treated by the blank sample group is intact and smooth, while the bacterial cell membrane treated by the antibacterial hydrogel generally shrinks, deforms, or even breaks.
These methods have their own advantages and disadvantages. The appropriate method can be selected to test the antibacterial gel according to specific needs and experimental conditions. At the same time, in order to ensure the accuracy and reliability of the test results, the operation should be carried out in strict accordance with relevant standards and regulations.

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