Complanatus glycosides detectionHow to apply for a report? What items need to be tested? We will conduct testing and evaluation in strict accordance with the standards. We can also provide personalized testing plans and reports according to your needs.

The detection method of complanatus glycoside mainly adopts high-performance liquid chromatography (HPLC). The following is a detailed introduction to a typical HPLC detection method for complainants glycoside:

I. Instruments and materials
High-performance liquid chromatography
Chromatographic column: such as ZORBAX EXTEND C18 (4.6mm×250mm, 5μm) or other suitable C18 chromatographic columns
Methanol, acetonitrile, phosphoric acid, and other chromatographic reagents
Compastragalus complainants glycoside reference
Test sample (Compastragalus complainants extract or related medicinal materials)
II. Chromatographic conditions
Mobile phase: acetonitrile-water-phosphoric acid, the ratio is usually 20:80:0.2 (some reports are 21:79:0.1% or other ratios), which needs to be optimized according to experimental conditions.
Detection wavelength: 266nm or 267nm (selected according to the instrument and experimental conditions)
Flow rate: generally 1.0mL/min
Column temperature: room temperature or set according to experimental conditions
Injection volume: generally 10μL
III. Preparation of test solution
Take an appropriate amount of Compastragalus complanatus powder (sieved), weigh it accurately, and place it in a stoppered conical flask.
Add an appropriate amount of 60% ethanol or other suitable solvent, weigh it, and heat it under reflux for a period of time (such as 1 hour).
After cooling, weigh it again and make up the lost weight with the same solvent.
After shaking, filter and take the filtrate as the test solution.
IV. Preparation of reference solution
Take an appropriate amount of comparative glycoside reference, accurately weigh it, and add 60% ethanol or another suitable solvent to make a solution of a certain concentration, such as a solution containing 15μg complanate glycoside per 1 mL.
V. Determination steps
Inject the reference solution and the test solution into the high-performance liquid chromatography for determination.
Separate and detect according to the set chromatographic conditions.
Record the chromatogram, and calculate the content of complanatine glycoside in the test solution with the chromatographic peak of the reference solution as the reference.
VI. Precautions
During the experiment, the experimental conditions, such as temperature, humidity, light, etc., should be strictly controlled to avoid affecting the experimental results.
The reagents and solvents used should comply with the relevant standards and expired or unqualified reagents and solvents should be avoided.
When preparing the test solution and the reference solution, they should be accurately weighed and measured to avoid errors.
The selection and use of the chromatographic column have an important influence on the experimental results. The appropriate chromatographic column should be selected according to the experimental conditions and the characteristics of the medicinal materials.
The content of complanatine in Astragalus complanatus can be accurately determined by high-performance liquid chromatography, which provides an important basis for the quality control and efficacy evaluation of Astragalus complanatus. At the same time, this method has the advantages of good separation effect, high sensitivity, and good reproducibility, and is suitable for the quantitative analysis of comparative glycoside.

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