Lectin detectionHow to apply for a report? What items need to be tested? We will conduct testing and evaluation in strict accordance with the standards. We can also provide personalized testing plans and reports according to your needs.

There are many methods for lectin detection, and the choice of these methods usually depends on the type and source of the lectin and the specific needs of the detection. The following are some common lectin detection methods:

1. Plant lectin detection method

Sugar complex method

Principle: Lectins can specifically bind to sugars, glycoproteins, or sugar complexes and quantitatively determine their content. Sugar complexes interact with the multivalent sugar binding sites of lectins, and the resulting conjugates precipitate under high salt concentrations.

Operation: The conjugates are separated by centrifugal filtration. In the earliest times, sugar complexes were labeled with radioactive isotopes, and the content of plant lectins was determined by detecting the intensity of the radioactive label in the precipitate.

Enzyme-linked immunosorbent (ELISA) method

Principle: Using the double antibody sandwich method, specimens, standards, and HRP-labeled detection antibodies are added to the microwells pre-coated with plant lectin capture antibodies in sequence. After incubation and washing, the substrate TMB is used for color development. TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with the content of plant lectins.
Operation: Use an enzyme marker to measure the absorbance value at a specific wavelength, and calculate the lectin content in the sample by the standard curve method.
Hemagglutination method
Principle: Use the property of lectins to agglutinate red blood cells (such as human and animal red blood cells) for detection.
Operation: Under specific conditions, mix the sample to be tested with red blood cells to observe whether agglutination occurs, so as to determine the presence and activity of agglutinins.
II. Hemagglutinin detection method (mainly used in the medical field)
Anti-human globulin test
Used to detect whether there is autoimmune hemolytic anemia to determine whether further hematological examination is needed.
Operation: Draw blood samples through veins, separate red blood cells after centrifugation under sterile conditions, and add specific reagents to observe whether there is agglutination.
Red blood cell morphological analysis
It can evaluate whether the indicators such as red blood cell size and shape are abnormal, which is helpful for diagnosing hereditary hemolytic anemia.
Operation: Collect peripheral blood smears, and use a microscope to observe and record the number and morphological characteristics of red blood cells.
Routine blood test
Including white blood cell count, red blood cell count, platelet count, and other items, it can preliminarily determine whether there are problems such as infection or bleeding.
Operation: Usually, blood is drawn from the elbow vein and sent to the laboratory for analysis immediately after sampling.
Coagulation function test
It is used to evaluate blood coagulation ability, which is crucial for guiding blood transfusion and pre-and post-operative management.
Operation: Generally, venous blood is drawn in a fasting state, and blood coagulation is observed within a specified time.
Determination of whole blood viscosity
It can reflect blood fluidity and concentration, which is helpful for cardiovascular disease risk assessment.
Operation: A certain amount of blood sample is drawn through a specific instrument, and its viscosity is measured at a certain temperature.
3. High-performance liquid chromatography (HPLC) method
Application: Especially suitable for the determination of lectins in edible fungi.
Principle: Through high-performance liquid chromatography technology, combined with ultrafiltration, ion exchange chromatography, and other technologies, lectins are separated and purified for quantitative analysis.
Advantages: high accuracy, high sensitivity, and good reproducibility.
IV. Other methods
Slide agglutination method: a conventional qualitative test method, the principle is to use known antibodies to detect unknown antigens, often used to identify bacterial species and blood types.
Tube agglutination method: a quantitative test method, which often uses known antigens to detect the presence and content of corresponding antibodies in serum, and is often used to assist in the diagnosis of certain infectious diseases and conduct epidemiological surveys.
In short, there are many ways to detect agglutinins, and the most suitable method should be selected according to the specific situation. During the test process, the operating procedures should be strictly followed to ensure the accuracy and reliability of the test results.

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