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Detailed description

Food testing items GB 5009.24-2016 National Food Safety Standard - Determination of aflatoxin M grou

Detection standard

  • Detection specification


This standard specifies the determination and method of aflatoxin M and aflatoxin M2 (hereinafter referred to as AFT M and AFT M2) in food The first method is isotope dilution liquid chromatography tandem mass spectrometry, which is suitable for the determination of AFT M: and AFT M2 in milk, dairy products, and special dietary foods containing milk The second method is high-performance liquid chromatography, with the same scope of application as the first method The third method is the enzyme-linked immunosorbent assay (ELISA) screening method, which is suitable for the screening and determination of AFTM in milk, dairy products, and special dietary foods containing milk

Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry


The aflatoxin M and aflatoxin Mz in the sample were extracted with methanol aqueous solution, the supernatant was diluted with water or phosphate buffer, purified and enriched by an immunoaffinity column, and the purified solution was concentrated. After constant volume and filtration, they were separated by liquid chromatography, detected by tandem mass spectrometry, and quantified by isotope internal standard method

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